I-Novel coronavirus pneumonia ilethele abantu ukuqonda okukhulu ngokulimala okukhulu kwezifo ezithathelwanayo emphakathini wesintu kusukela ngoDisemba nyakenye. Ukhiye wokuhlela ukutheleleka nokulawula okuphelele ukwenza kahle ekuvikeleni nasekulawuleni ubhadane futhi uhlanze indawo yokunakekela iziguli.
Ocwaningweni lwelabhoratri, ubuchwepheshe bokukhanya bokukhanyisa okuphezulu kufakazelwe ukuthi kuyasebenza ekunciphiseni amagciwane nasekubulaleni inzalo. Ukuze kuqhutshekwe nokutadisha ukusebenza kahle kwezemvelo kanye nokwenzeka kwalobu buchwepheshe bokuxhumana, i-Professional Research Institute yenze ucwaningo lwezinyanga ezine eQueen's Hospital eNorth London, e-UK.

Lolu cwaningo lwenziwa kusukela ngoJulayi 2014 kuya kuNovemba 2014. Kukhethwe amawadi angama-40 okuhlukaniswa esibhedlela njengamasampula ocwaningo. Ngemuva kokukhishwa kweziguli esibhedlela, zahlanzwa ngesandla ngesisombululo se-hypochlorite, ekugcineni zafakwa inzalo ngemishini ye-pulse ultraviolet disinfection. Ngemuva kwalokho, ochwepheshe bathatha amasampula amabhaktheriya e-aerobic, badalula i-agar plate egonyiwe endaweni enganakekelwa iziguli, futhi bahlola i-pulse ultraviolet disinfection Umphumela wemishini ku-microorganism nayo izorekhoda imizwa yabasebenzi basesibhedlela ngokusetshenziswa kwemishini.

Indlela yokuhlola

Ithimba labacwaningi lenze ucwaningo lokuqhathanisa ukusampula izindawo ezinhlanu ezixhumana kakhulu (imibhede yombhede, amatafula e-pallet, izibambo zangasese, izihlalo zangasese nezibambo zompompi wokugezela) ngaphambi kokubulala amagciwane, ngemuva kokubulala amagciwane okufakelwayo nangemva kokubulala amagciwane imishini ye-ultraviolet disinfection yokuhlola ukusebenza kahle okokusebenza kwe-ultraviolet disinfection imishini ekunciphiseni ukungcoliswa kwemvelo ezindaweni ezihlukanisiwe zeziguli ezikhishelwe ezocansi.

Ukukhethwa kwesampula

Khetha izigceme (amakamelo ayi-6 ngeyunithi ngayinye) kusuka kumayunithi wokuhlola okwelashwa okunamandla. Ilabhorethri inqunywa yi-database yokuvikela nokutheleleka kokutheleleka ekusetshenzisweni kwabasebenzi bokuvikela ukutheleleka nokulawula. Indlela yokukhetha yelabhorethri imi kanje:

(1) Kufanele kube yigumbi elilodwa;

(2) Kufanele uhlale okungenani amahora angama-48;

(3) Kufanele isuswe ngosuku olufanayo lokuqoqwa kwesampula;

(4) Kufanele isetshenziswe njengegumbi lokuhlukanisa elingaxhumana nabo.

Inqubo yokuhlola

Amasampula ayisisekelo we-microbiological aqoqwa ngemuva kokukhishwa, kepha ngaphambi kokuhlanzwa okujwayelekile. Izindawo ezinhlanu zokuxhumana ezivama kakhulu zaqala ukwenziwa isampula yi-trypsin soybean agar contact plate (i-Oxford, iBasingstoke, UK) enobubanzi obungu-5 mm;

Abahlanza izibhedlela basebenzisa i-1000 ppm (0.1%) i-chlorine disinfectant (activalum

Futhi; I-Ecolab, Cheshire, UK) yokuhlanza okujwayelekile kwetheminali kanye nesampuli yesibili;

Igumbi lalikhanyiselwa irobhothi elikhuculula amagciwane. Amaphuzu amathathu akhethwe ewadini ngayinye: izinhlangothi ezimbili zombhede nendawo yokugezela. Iphuzu ngalinye lalikhanyiselwa i-5min. Ngemuva kokubulala amagciwane, kwaqoqwa amasampula ezindaweni ezifanayo ezi-5 ukuqedela isampula lokugcina.

Isampula eliqoqiwe libekwa endaweni ekhethiwe kuqala ukuvimbela noma ikuphi ukuphambuka noma ushintsho lwendlela yokuhlanza. Ngemuva kokuqoqwa kwesampula, i-trypsin soybean agar plate plate ibuyele elabhorethri, ikhuliswe emoyeni kuma-37 ° C amahora angama-48, yabalwa futhi yaqopha inani lamayunithi akha amakoloni (CFU).

Ukuhlaziywa kwedatha

Igumbi elilodwa linqatshiwe ngoba belungekho ulwazi mayelana nokutheleleka kwemishini ye-pulse, futhi isampula lehliselwa emakamelweni angama-39.

Ekuqaleni, ingxenye enkulu kunazo zonke (93%) yamakamelo angcolisiwe yabonwa ebusweni besitimela sombhede, esancishiswa saba ngu-36% ngemuva kokuhlanzwa ngesandla kanye no-7% ngemuva kokubulawa magciwane yirobhothi le-ultraviolet disinfection.

umphumela wokuhlola

Ngemuva kokuthi irobhothi lenziwe inzalo yi-UV ephephuliwe, ukungcoliswa kwamagciwane ku-CFU kwehle ngama-78.4%, ama-91% angaphansi kwezinga lokuqala le-bioburden. I-CFU yama-MDROs kupuleti yezipikili yehliswe ngo-5 log. Ngophenyo nocwaningo, opharetha bemishini banelisekile ngenduduzo yomkhiqizo.

isiphetho

Kusetshenziswa imishini yokwakha amagciwane engathintwa emisha emisha kuyo yonke imikhakha yezokwelapha ukuqinisekisa ukuhlanzeka kwemvelo yesibhedlela. Ngalesi silingo, sithole ukuthi:
1. Inhlanganisela yokuhlanza okwenziwe ngamakhemikhali nokubulala amagciwane kwehlulekile ukususa ngempumelelo ukungcoliswa kwamagciwane emvelweni.
2. Ngemuva kokusebenzisa imishini ye-pulse ultraviolet disinfection, ukungcoliswa komhlaba kwegumbi lokuhlukaniswa kwehliswe kakhulu.